Journal: Immunity

Article Title: Lung γδ T cells mediate protective responses during neonatal influenza infection that are associated with Type-2 immunity

doi: 10.1016/j.immuni.2018.07.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The stained cells were resuspended in RNAse inhibitor containing sort buffer (RNAsin, Promega, 200U/μl) and sorted by gating on γδTCR + CD3 + cells directly into a 384-well PCR plate that had been preloaded with 1μl of reverse transcription reaction mix [1X SuperScript VILO Reaction Mix (Thermo Fisher), SuperScript VILO enzyme and 1X0.1% NP40 (Thermo Fisher)] with a sorter (Model SY3200, Sony Biotech Synergy sorter, Sony Biotech, San Jose, CA).

Techniques: Control, Virus, Plasmid Preparation, Recombinant, Cell Stimulation, Protease Inhibitor, Blocking Assay, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Sequencing, Software

Journal: Advanced Functional Materials

Article Title: Tumor‐On‐A‐Chip Model Incorporating Human‐Based Hydrogels for Easy Assessment of Metastatic Tumor Inter‐Heterogeneity

doi: 10.1002/adfm.202315940

Figure Lengend Snippet: Figure 5. Gene and protein biomarkers expression depending on tumor metastatic ability. a) Relative gene expression of VEGFA, COL1A1, MMP2, and MMP9 in the (i) low-metastatic and (ii) high-metastatic models for the different culture conditions, normalized to the static culture. b) Comparison of low- and high-metastatic relative gene expression of (i) VEGFA, (ii) COL1A1, and (iii) MMP2, for each culture condition. c) ELISA quantification of VEGFA protein secretion at days 3, 4, and 7. Statistical significance between different culture conditions, for the same time-point, is represented with a solid line. Unless otherwise stated, time-dependent significance in each culture condition and model is statistically different. d) Confocal images of Ezrin expression (green) in the low-metastatic model, at 7 days of culture. Actin filaments and nuclei are stained in red and blue, respectively. Scale bar: 200 μm. Data are presented as mean ± SD (n ≥3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, ns: not significant.

Article Snippet: The samples were incubated with primary mouse monoclonal anti-human Ezrin antibody (1:50 in 5% FBS (v/v) in PBS, 3C12, Santa Cruz Biotechnology, USA) at 4 °C for 3 days, followed by PBS washing for 1 h, and then incubated with the secondary antibody goat antimouse Alexa Fluor 488 (1:100 in 5% FBS (v/v) in PBS, Thermo Fisher Scientific, USA) at 4 °C for 3 days.

Techniques: Expressing, Gene Expression, Comparison, Enzyme-linked Immunosorbent Assay, Staining

Journal: Cell reports

Article Title: A FLCN-TFE3 Feedback Loop Prevents Excessive Glycogenesis and Phagocyte Activation by Regulating Lysosome Activity

doi: 10.1016/j.celrep.2020.01.042

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SuperScript IV VILO Master Mix with ezDNase Enzyme , Thermo Fisher Scientific , Cat#11766050.

Techniques: Purification, Blocking Assay, Recombinant, Transfection, Flow Cytometry, Magnetic Beads, Lysis, Extraction, SYBR Green Assay, Staining, shRNA, Sequencing, Plasmid Preparation, Software